目的 分析不同种源太子参中化学成分的差异。方法 采用超高效液相-串联四极杆飞行时间高分辨质谱(UPLC-Triple TOF-MS/MS)技术对不同种源太子参24批样品进行测定,通过多级串联质谱分析,对其质谱数据进行峰匹配、峰对齐、滤噪处理等进行特征峰提取;用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA) 进行数据处理;通过一级质谱精确质荷比和二级质谱碎片信息,结合软件数据库搜索及相关文献进行成分鉴别。结果 不同种源太子参样品间的化学组成得到明显区分;初步筛选并鉴定出不同种源太子参间21种差异显著的化学成分,且11种共有差异化学成分呈现不同的变化规律。结论 为揭示种源遗传对太子参代谢物合成积累的影响规律提供基础资料。
Abstract
OBJECTIVE To analyze the differences in chemical composition of Pseudostellariae Radix from different germplasms. METHODS Tweny-four batches of samples were determined by UPLC-Triple TOF-MS/MS. Characteristic peaks were extracted through treatment of mass spectrometry data including peak matching, peak alignment and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. Component identification was carried out based on the mass spectrometry accurate mass and secondary mass spectrometry fragmentation information combined with database search and literature. RESULTS The differences among samples of Pseudostellariae Radix of different germplasms were distinguishable. Twenty-one chemical compositions with significant differences were screened, among which 10 common chemical compositions showed different changing laws. CONCLUSION This study provides experimental data for revealing the laws of Pseudostellariae Radix metabolites from different germplasms.
关键词
太子参 /
超高效液相-串联四极杆飞行时间高分辨质谱 /
种源资源 /
化学成分
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Key words
Radix Pseudostellariae /
UPLC-triple TOF-MS/MS /
germplasm resource /
chemical composition
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中图分类号：
R917
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参考文献
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脚注
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基金
国家自然科学基金资助项目(81274016;81473312);国家科技支撑计划资助项目(2011BAI13B04);江苏高校优势学科建设工程资助项目(ysxk-2014)
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